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NEB M0544L NEBNext Ultra II Q5 Master Mix 250rxns

  • 商品编号:01.NEB.M0544L
  • 货号:01.NEB.M0544L
  • 品  牌:NEB
  • 市场价: ¥3559.00
  • 销售价: ¥3559.00
  • 广州仓库:无现货  上海仓库:无现货  北京仓库:无现货
    (库存情况仅供参考,请来电确认准确的现货情况)
  • 预计到货期:7
  • 本商品仅供科研之用,不可用于临床

The NEBNext Ultra II Q5® Master Mix is a new formulation of Q5 DNA polymerase that has been optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries. This new formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.

The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has the highest fidelity available (> 100-fold higher than that of Taq DNA Polymerase and ~12-fold higher than that of Pyrococcus furiosus (Pfu) DNA Polymerase), resulting in ultra-low error rates.

The NEBNext Ultra II Q5 Master Mix is an aptamer-based hot start formulation that allows convenient room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. NEBNext Ultra II Q5 Master Mix is also included in the NEBNext Ultra II DNA Library Prep Kit for Illumina®

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